Composite

Part:BBa_K899003

Designed by: Andreas Kjær   Group: iGEM12_SDU-Denmark   (2012-09-22)

Reporter system for FFT (BBa_K899001)

Reporter system for characterization of fructan fructan fructosyltransferase (FFT) gene transcription, with green flourescent protein (GFP) as reporter and lacl as promoter. This construct was designed with the promoter BBa_R0010 in order to control transcription. We suspect transcription of 1-FFT to greatly inhibit bacterial growth with the production of plant inulin. LacI protein and CAP can succesfully inhibit transcription of both genes to allow the bacteria to grow. With the addition of IPTG to the medium, LacI will be inhibited and transcription can resume.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 721
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 575
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2725


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Categories
Parameters
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